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Image Search Results
Journal: PLoS ONE
Article Title: CD133: Enhancement of Bone Healing by Local Transplantation of Peripheral Blood Cells in a Biologically Delayed Rat Osteotomy Model
doi: 10.1371/journal.pone.0052650
Figure Lengend Snippet: (A) Tube length evaluation for CD34 (+)/(−) and CD133(+)/(−) cells normalized to the tube length measured in the control group together with all PBMCs, note the significant difference in tube length between CD133(+) and CD133(−), n = 8, * p = 0.018; (B) Tube formation of CD133(+) and CD133(−) respectively, note the more pronounced tube formation in the CD133(+) fraction.
Article Snippet: CD34(+) and CD133(
Techniques: Control
Journal: PLoS ONE
Article Title: CD133: Enhancement of Bone Healing by Local Transplantation of Peripheral Blood Cells in a Biologically Delayed Rat Osteotomy Model
doi: 10.1371/journal.pone.0052650
Figure Lengend Snippet: The gene expression of (A) b-FGF, (B) PDGFa were analyzed with quantitative PCR and normlaized to the expression of Tbp. Note the more pronounced gene expression of b-FGF and PDGFa in CD133(+) cells, n = 5, * p = 0.009.
Article Snippet: CD34(+) and CD133(
Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Expressing
Journal: PLoS ONE
Article Title: CD133: Enhancement of Bone Healing by Local Transplantation of Peripheral Blood Cells in a Biologically Delayed Rat Osteotomy Model
doi: 10.1371/journal.pone.0052650
Figure Lengend Snippet: ( A ) 3D reconstructions from µCT scans from one representative animal after 2, 4, and 6 weeks of the three groups are shown. In the CD133(+) group (left) mineralized tissue was formed at the endosteal as well as at the periosteal regions. After 42 days the gap had almost been bridged; a thin layer of radiolucent tissue still separated the bone ends. In the ABC group (middle) only very little callus formation was observed, appearing between the 28th and 42nd day after the osteotomy. No bridging but a cap-formation was observed, sealing the medullary canal. In the CD34(+) group (right) bone formation was further progressed than in the ABC group. After 6 weeks however, bridging did not occur and the medullary canal was closed at the proximal end. (Resolution 28 µm; scale: 2 mm). ( B ) Bridging score for the ABC group is lower compared with the bridging in the CD34(+) or CD133(+) group. The bridging score for the CD34(+) group showed better results than the ABC group but lesser results compared with the CD133(+). 5 areas in the osteotomy gap were investigated, 12, 3, 6, and 9 o'clock position and the centre region. The sum of bridged areas for the healing course is indicated in the table.
Article Snippet: CD34(+) and CD133(
Techniques:
Journal: PLoS ONE
Article Title: CD133: Enhancement of Bone Healing by Local Transplantation of Peripheral Blood Cells in a Biologically Delayed Rat Osteotomy Model
doi: 10.1371/journal.pone.0052650
Figure Lengend Snippet: An increase of callus volume (CV, [mm3]), as well as bone mineral contend (BMC, [mg]) on the basis of total mineral density of the callus was observed over time. Both parameters were significantly higher in the CD133(+) group when compared to the ABC group. Note that the differences occur not until after the 28th day and are most pronounced after the 42nd day. Comparing CV and BMC between the CD133(+) group and the CD34(+) group markedly higher values were found in the CD133(+) group. (CV: total volume, BMC: bone mineral content, wks: weeks); (CD133(+) group = grey; CD34(+) group = checkered; ABC (autologous blood clot) group = white).
Article Snippet: CD34(+) and CD133(
Techniques:
Journal: PLoS ONE
Article Title: CD133: Enhancement of Bone Healing by Local Transplantation of Peripheral Blood Cells in a Biologically Delayed Rat Osteotomy Model
doi: 10.1371/journal.pone.0052650
Figure Lengend Snippet: ( A ) Photomicrographs of histological sections 6 weeks post-surgery of the CD133(+) (left) and the ABC (right) group (Movat Pentachrome staining); In the CD133(+) group the osteotomy gap was predominantly filled with spongy mineralized tissue formations. A thin layer of hyaline cartilage (green) between the bone ends had not yet been mineralized. In the ABC group almost only fibrous tissue was found, completely lacking any cartilage or bone formation; (ABC: autologous blood clot group; CD133(+): CD133 cell treated group); cortical bone/yellow; bone marrow/dark red; cartilage/green; muscle/red . ( B ) Photomicrographs of immune histological sections 6 weeks post-surgery of the CD133(+) (left) and the ABC (right) group (factor VIII staining); In the CD133(+) group quite dense vessel formations (red) were observed, especially in the endostal region at the zone where the hyaline cartilage formations are mineralized. (ABC: autologous clot group; CD133: CD133+ cell treated group) (Scale bar: 1 mm).
Article Snippet: CD34(+) and CD133(
Techniques: Staining